streptomyces avermitilis meaning in English
及营养条件对阿佛曼菌
Examples
- Effect of avec gene deletion on avermectins production in streptomyces avermitilis
基因缺失对产素调控的影响 - The transformants were cultivated and the harvested cells were used in the procedure of conjugation by which the recombinant plasmid pid03 and pc05 were introduced into streptomyces avermitilis s - 2 respectively
经过在mym平板上的传代和抗性标记的筛选分别得到了同源双交换的菌株aved24和avecg 。 - In order to obtain a dna fragment containing aved gene , 5 ' end primer and 3 ' end primer , consisting of 18 and 20 nucleotides respectively , were designed and synthesized . a 2 . 2 kb dna fragment containing aved gene was obtained by pcr when genomic dna of streptomyces avermitilis s - 2 was used as a template
为了得到aved基因片段，首先根据已知的阿维菌素生物合成基因簇的核苷酸序列，设计并合成了由18和20个核苷酸组成的5端和3端引物；以streptomycesavermitiliss - 2基因组dna为模板，经pcr扩增得到了2 . 2kb的含有aved基因的dna片段。 - In addition to avermectins , s . avermitilis produces oligomycin , a strongly toxic compound . gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73 , the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin . olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover
本研究以产阿维菌素b和寡霉素的阿维链霉菌cz8 - 73为出发菌株，构建了基因缺失载体pxl05 ，并将其转入cz8 - 73中，通过缺失载体和染色体之间的同源双交换，对染色体上长达90kb的寡霉素聚酮合酶( pks )基因簇( olma )进行了缺失。 - In this study , the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering . insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins . when avec gene was inactivated by the same way , four " 1 " components were lost and only " 2 " components , the potential precursor of ivermectin , were accumulated
将该基因簇中的aved基因通过插入外源的安普霉素抗性基因片段使其失活，导致发酵产物中4个a组分(不需要的组分)的消失；将基因簇中的avec基因通过同样手段，使其失活，导致发酵产物中4个“ 1 ”组分的消失，而主要积累“ 2 ”组分(进一步改造可成为伊维菌素的前体b _ 2组分) 。
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